Journal: Drug Discovery Today

Article Title: Intranasal vaccines for SARS-CoV-2: From challenges to potential in COVID-19 management

doi: 10.1016/j.drudis.2021.07.021

Figure Lengend Snippet: Nasal sprays for COVID-19 treatment.

Article Snippet: Inhaled mRNA antibodies , Neurimmune, and Ethris , SNIM®RNA technology , mRNA-encoded, neutralising anti-SARS-CoV-2 antibodies , Phase I imminent , Germany , .

Techniques:

Journal: Frontiers in cell and developmental biology

Article Title: Inhibiting Heat Shock Protein 90 Protects Nucleus Pulposus-Derived Stem/Progenitor Cells From Compression-Induced Necroptosis and Apoptosis.

doi: 10.3389/fcell.2020.00685

Figure Lengend Snippet: FIGURE 1 | Compression induced necrotic cell death of NPSCs. (A) IHC staining of Tie2 for marking NPSCs in non-degenerated (37 years old, male, grade II) and degenerated (43 years old, male, grade IV) human NP tissues (original magnification: ×400). (B) Cell viability of NPSCs examined by CCK-8 assays. (C) The relative release of LDH at different time points. (D) Representative dot plots of PI staining obtained from flow cytometry analysis of NPSCs. (E) The statistical analysis of PI positive ratio of NPSCs. (F) The morphological ultrastructural appearance of NPSCs observed by TEM. The NPSCs exposed to 48 h of compression displayed necrotic morphological changes, such as severe vacuolation, swelling of organelles and disruption of the plasma membrane. The data were expressed as mean ± SD from at least three independent experiments, and they were analyzed by a two-tailed t-test or ANOVA. (**P < 0.01, ***P < 0.001 vs. 0 h).

Article Snippet: Cells were then permeabilized with 0·5% Triton X-100 (Beyotime) for 15 min at room temperature (for staining of Tie2, the permeabilization was not performed), and blocked with goat serum albumin for 1 h. Next, samples were washed with Frontiers in Cell and Developmental Biology | www.frontiersin.org 3 August 2020 | Volume 8 | Article 685 PBS and incubated with the mixture of mouse anti-HSP90 antibody (1:200, Santa Cruz Biotechnology) and rabbit antiphosphorylated MLKL (P-MLKL) antibody (Ser358, 1:200, Affinity Biosciences, OH, United States), the rabbit anti-HSP70 antibody (1:500, ABclonal, Wuhan, China), or rabbit anti-Tie2 antibody (1:100, Proteintech Group, Wuhan, China) at 4◦C overnight, followed by incubation with fluorophore-conjugated secondary antibody (1:200, Proteintech Group).

Techniques: Immunohistochemistry, CCK-8 Assay, Staining, Cytometry, Disruption, Clinical Proteomics, Membrane, Two Tailed Test

Journal: Frontiers in cell and developmental biology

Article Title: Inhibiting Heat Shock Protein 90 Protects Nucleus Pulposus-Derived Stem/Progenitor Cells From Compression-Induced Necroptosis and Apoptosis.

doi: 10.3389/fcell.2020.00685

Figure Lengend Snippet: FIGURE 9 | Inhibiting HSP90 attenuated the exhaustion of NPSCs in vivo. Hematoxylin and eosin staining (original magnification: ×25) and the IHC staining of Tie2 (original magnification: ×25 and 400) for labeling endogenous NPSCs of IVDs.

Article Snippet: Cells were then permeabilized with 0·5% Triton X-100 (Beyotime) for 15 min at room temperature (for staining of Tie2, the permeabilization was not performed), and blocked with goat serum albumin for 1 h. Next, samples were washed with Frontiers in Cell and Developmental Biology | www.frontiersin.org 3 August 2020 | Volume 8 | Article 685 PBS and incubated with the mixture of mouse anti-HSP90 antibody (1:200, Santa Cruz Biotechnology) and rabbit antiphosphorylated MLKL (P-MLKL) antibody (Ser358, 1:200, Affinity Biosciences, OH, United States), the rabbit anti-HSP70 antibody (1:500, ABclonal, Wuhan, China), or rabbit anti-Tie2 antibody (1:100, Proteintech Group, Wuhan, China) at 4◦C overnight, followed by incubation with fluorophore-conjugated secondary antibody (1:200, Proteintech Group).

Techniques: In Vivo, Staining, Immunohistochemistry, Labeling